T9822
Sigma
Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse
clone B3, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD03792435 |
Description
| Immunogen | purified sea urchin (Lytechinus pictus) sperm axonemal proteins. |
| Specificity | Epitope recognized by the antibody is localized in the C-terminal region of α- and β-tubulins (the glutamylated motif at amino acids 445-457 of α-tubulin). Useful in studies involving the inhibition of flagellar motility in permeabilized sperm models. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide |
Properties
| antibody form | purified immunoglobulin |
| clone | B3 (monoclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~50 kDa |
| species reactivity | mouse, Oxyrrhis marina (dinoflagellate), sea urchin (Lytechinus pictus and three others.), chicken, human, bovine, rabbit, rat, monkey |
| application(s) | immunoblotting: 1-2 μg/mL using whole extract of cultured normal rat kidney (NRK) cells |
| immunocytochemistry: suitable | |
| indirect ELISA: suitable | |
| microarray: suitable | |
| isotype | IgM |
| Gene Information | human ... TUBA4A(7277), TUBB(203068) |
| rat ... Tuba1a(64158) | |
| mouse ... Tuba1a(22142), Tubb2b(73710) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 2 |
Related Products
References
| reference | Gagnon, C., et al. J. Cell Sci. 109, 1545, (1996) Abstract |
| Bulinski, J., et al., Normal and prostate cancer cells display distinct molecular profiles of α-tubulin posttranslational modifications. Prostate 66, 954-965, (2006) Abstract | |
| Janke, C., et al., Loss of α-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function Proc. Natl. Acad. Sci. U. S. A. 104, 3213-3218, (2007) Abstract | |
| Moreno, F., et al., A Mutation in CCDC50, a Gene Encoding an Effector of Epidermal Growth Factor–Mediated Cell Signaling, Causes Progressive Hearing Loss Am. J. Hum. Genet. 80, 1076-1089, (2007) Abstract |
